| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hering, Tanner A (18)
|
NCSGSA |
50.57 |
52.58 |
2.01 |
| 2 |
Hering, Flinn M (16)
|
NCSGSA |
52.39 |
55.11 |
2.72 |
| 3 |
Holder, Nathaniel D (16)
|
NCEAC |
51.82 |
55.22 |
3.40 |
| 4 |
Mikuta, Christopher R (18)
|
NCSGSA |
53.02 |
55.98 |
2.96 |
| 5 |
Cantrell, Eric J (17)
|
NCSGSA |
53.96 |
57.05 |
3.09 |
| 6 |
Maycock, Julian L (16)
|
NCSGSA |
54.51 |
57.22 |
2.71 |
| 7 |
Keaney, Reilly E (16)
|
NCSGSA |
53.05 |
58.19 |
5.14 |
| 8 |
Schilling, Christopher A (13)
|
NCSGSA |
1:04.16 |
1:01.62 |
-2.54 |
| 9 |
Hagler, Austin G (16)
|
NCSTAR |
1:02.67 |
1:02.16 |
-0.51 |
| 10 |
Austin, Thaddeus E (12)
|
NCEAC |
1:05.10 |
1:03.47 |
-1.63 |
| 11 |
Cartagena, Aidan C (13)
|
NCEAC |
1:04.95 |
1:06.26 |
1.31 |
| 12 |
Holian, Tanner J (14)
|
NCSGSA |
1:03.55 |
1:07.39 |
3.84 |
| 13 |
Rolland, Brady M (14)
|
NCSTAR |
1:12.89 |
1:07.55 |
-5.34 |
| 14 |
Mann, Spencer J (13)
|
NCEAC |
1:06.42 |
1:08.09 |
1.67 |
| 15 |
Burton, Bence W (13)
|
NCEAC |
1:06.59 |
1:09.00 |
2.41 |
| 16 |
Glebus, Christopher P (15)
|
NCSGSA |
1:06.47 |
1:09.67 |
3.20 |
| 17 |
Brooks, Nicholas R (11)
|
NCEAC |
1:06.98 |
1:10.96 |
3.98 |
| 18 |
Burton, Tyler A (11)
|
NCEAC |
1:10.38 |
1:13.58 |
3.20 |
| 19 |
Clark, Quintin S (13)
|
NCSTAR |
1:10.38 |
1:14.00 |
3.62 |
| 20 |
Powell, David A (12)
|
NCEAC |
1:36.97 |
1:24.19 |
-12.78 |
| 21 |
Mitchell, Micah J (11)
|
NCEAC |
1:17.45 |
1:24.97 |
7.52 |
| 22 |
Rossato, Finley M (11)
|
NCSGSA |
|
1:25.00 |
- |
| 23 |
McGonigal, Finn M (12)
|
NCSTAR |
1:50.24 |
1:26.33 |
-23.91 |
| 24 |
Teoh, Wesley P (11)
|
NCSTAR |
|
1:31.52 |
- |
| 25 |
Willis, Berkley S (10)
|
NCEAC |
1:30.32 |
1:39.67 |
9.35 |
|
Nunn, Bo B (9)
|
NCEAC |
1:41.62 |
DQ |
- |
|
Smelzer, Peter B (16)
|
NCSGSA |
55.59 |
NS |
- |