| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Patterson, Lauren E (10)
|
NCWST |
32.66 |
31.60 |
-1.06 |
| 2 |
Brooks, Alexandria I (10)
|
NCSFSC |
34.63 |
31.65 |
-2.98 |
| 3 |
Scoggins, Avery L (10)
|
NCTRY |
34.80 |
34.15 |
-0.65 |
| 4 |
McGonigal, Maeren R (10)
|
NCSTAR |
|
34.44 |
- |
| 5 |
Ramseur, Reece E (10)
|
NCSFSC |
39.66 |
34.71 |
-4.95 |
| 6 |
Horner, AnnaClaire C (10)
|
NCWST |
44.28 |
35.92 |
-8.36 |
| 7 |
Henderson, Ellie K (10)
|
NCSTAR |
37.09 |
36.41 |
-0.68 |
| 8 |
Baldwin, Sydney R (9)
|
NCEAC |
44.99 |
40.47 |
-4.52 |
| 9 |
Walton, Reese K (9)
|
NCUN-0 |
47.60 |
45.02 |
-2.58 |
| 10 |
Perez, Ella G (10)
|
NCSTAR |
46.95 |
45.77 |
-1.18 |
| 11 |
Fernando, Scarlett O (9)
|
NCSFSC |
|
46.59 |
- |
| 12 |
Reynolds, Anna Grace M (9)
|
NCSFSC |
49.39 |
48.35 |
-1.04 |
| 13 |
Richardson, Aryanna J (9)
|
NCSTAR |
46.90 |
49.45 |
2.55 |
| 14 |
Larson, Phoebe N (10)
|
NCTRY |
|
50.66 |
- |
| 15 |
Scorza, Kathryn M (10)
|
NCPAST |
55.72 |
55.02 |
-0.70 |
| 16 |
Spaulding, Halleigh I (10)
|
NCSTAR |
|
56.16 |
- |
| 17 |
Rasco, Emma A (9)
|
NCWST |
|
57.67 |
- |
|
Armstrong, Lillian G (10)
|
NCSFSC |
45.00 |
DQ |
- |
|
Cheves, Margaret B (9)
|
NCWST |
|
DQ |
- |
|
Mack, Kourie C (10)
|
NCSTAR |
|
DQ |
- |