| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Williams, Jordan G (11)
|
NCGCY |
36.48 |
36.05 |
-0.43 |
| 2 |
Lewis, Lauren E (11)
|
NCTYDE |
38.50 |
37.27 |
-1.23 |
| 3 |
Walls, Kate G (12)
|
NCEAC |
37.64 |
37.98 |
0.34 |
| 4 |
Brown, Keegan H (11)
|
NCTYDE |
38.85 |
39.06 |
0.21 |
| 5 |
Flowers, Charlotte W (12)
|
NCEAC |
44.09 |
40.34 |
-3.75 |
| 6 |
Holton, Nina M (12)
|
NCEAC |
44.26 |
40.64 |
-3.62 |
| 7 |
Shackelford, Alison R (12)
|
NCUN |
42.76 |
41.76 |
-1.00 |
| 8 |
Super, McKenna L (11)
|
NCGCY |
44.14 |
42.55 |
-1.59 |
| 9 |
Martin, Cameron A (11)
|
NCGCY |
44.21 |
43.38 |
-0.83 |
| 10 |
Welborn, Sophia S (11)
|
NCTYDE |
43.04 |
43.39 |
0.35 |
| 11 |
Mann, Kenzie B (12)
|
NCGCY |
41.35 |
43.61 |
2.26 |
| 12 |
Steele, Ava C (11)
|
NCTYDE |
42.18 |
43.82 |
1.64 |
| 13 |
Copeland, Addison O (11)
|
NCTYDE |
50.03 |
44.62 |
-5.41 |
| 14 |
Scott, Ashtyn H (12)
|
NCGCY |
46.33 |
45.19 |
-1.14 |
| 15 |
Coburn, Zoe E (12)
|
NCGCY |
49.40 |
45.49 |
-3.91 |
| 16 |
White, Kaley E (12)
|
NCGCY |
46.36 |
45.78 |
-0.58 |
| 17 |
Lackey, Elizabeth W (11)
|
NCSFSC |
45.65 |
46.29 |
0.64 |
| 18 |
Neighbors, Kaylie E (12)
|
NCSFSC |
46.08 |
46.66 |
0.58 |
| 19 |
Davenport, Sydney O (11)
|
NCEAC |
58.49 |
46.94 |
-11.55 |
| 20 |
Harrell, Emilee C (11)
|
NCEAC |
46.24 |
47.86 |
1.62 |
| 21 |
Howell, Iyana (11)
|
NCGCY |
48.27 |
48.60 |
0.33 |
| 22 |
Whitworth, Margaret E (12)
|
NCTYDE |
56.77 |
49.73 |
-7.04 |
| 23 |
Barnett, Anna H (11)
|
NCSFSC |
49.79 |
50.61 |
0.82 |
| 24 |
Snyder, Rebecca E (11)
|
NCEAC |
47.11 |
52.99 |
5.88 |
| 25 |
Clarida, Brianna S (12)
|
NCSFSC |
|
59.11 |
- |
|
Grosz, Lauren A (11)
|
NCGCY |
1:06.70 |
DQ |
- |
|
Van Noy, Jaylin L (12)
|
NCTYDE |
|
NS |
- |