| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morrow, Connor E (12)
|
NCUN |
26.47 |
26.39 |
-0.08 |
| 2 |
Harris, Dax C (12)
|
NCGCY |
26.23 |
26.80 |
0.57 |
| 3 |
Greene, Jonah R (12)
|
NCTYDE |
27.61 |
27.86 |
0.25 |
| 4 |
Fore, Daniel G (12)
|
NCTYDE |
28.09 |
28.51 |
0.42 |
| 5 |
Cayer, Connor N (11)
|
NCTYDE |
28.42 |
28.75 |
0.33 |
| 6 |
Sharpe, Sterling E (11)
|
NCGCY |
29.22 |
29.07 |
-0.15 |
| 7 |
Valliere, Alexander H (12)
|
NCTYDE |
28.62 |
29.28 |
0.66 |
| 8 |
Farmer, Zane M (12)
|
NCTYDE |
28.42 |
29.45 |
1.03 |
| 9 |
Kazel, Max B (12)
|
NCGOLD |
29.37 |
29.52 |
0.15 |
| 10 |
Murphy, Stratton T (12)
|
NCGCY |
28.38 |
29.99 |
1.61 |
| 11 |
Auman, Isaiah H (12)
|
NCUN |
30.60 |
30.42 |
-0.18 |
| 12 |
Cassidy, Austin W (12)
|
NCTYDE |
31.83 |
31.21 |
-0.62 |
| 13 |
Williams, Brett T (12)
|
NCEAC |
31.33 |
31.90 |
0.57 |
| 14 |
Schwier, Jacob (12)
|
NCGFSC |
30.99 |
32.14 |
1.15 |
| 15 |
Papaioannou, Christos (11)
|
NCTYDE |
31.24 |
32.74 |
1.50 |
| 16 |
Agarwala, Matthew B (12)
|
NCEAC |
|
32.95 |
- |
| 17 |
Hua, James R (12)
|
NCGCY |
42.10 |
33.45 |
-8.65 |
| 18 |
Hennessy, Ian T (12)
|
NCFISH |
|
35.42 |
- |
| 19 |
Bell, John J (12)
|
NCGCY |
36.36 |
35.70 |
-0.66 |
| 20 |
Rindal, Sam H (11)
|
NCGCY |
36.39 |
36.31 |
-0.08 |
| 21 |
Moye, Christopher S (12)
|
NCTYDE |
40.06 |
39.55 |
-0.51 |
| 22 |
Rondon, David B (11)
|
NCFISH |
39.60 |
40.88 |
1.28 |
| 23 |
Fleming, Robert T (11)
|
NCVACB |
40.91 |
41.19 |
0.28 |
| 24 |
Peterson, Alexander T (12)
|
NCVACB |
|
48.40 |
- |
|
Danhauer, Hayden T (11)
|
NCTYDE |
31.12 |
NS |
- |
|
Quaresma, David G (12)
|
NCGFSC |
29.84 |
NS |
- |