| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gardner, Harrison C (15)
|
NCHPSC |
51.14 |
50.88 |
-0.26 |
| 2 |
Haut, Isaac B (16)
|
NCHPSC |
51.41 |
51.98 |
0.57 |
| 3 |
Hall, Spencer M (15)
|
NCHPSC |
54.35 |
53.03 |
-1.32 |
| 4 |
Ulrich, Thomas M (17)
|
NCSTAR |
53.84 |
53.63 |
-0.21 |
| 5 |
Cox, John H (15)
|
NCSTAR |
56.67 |
55.57 |
-1.10 |
| 6 |
Ballesteros, Daniel A (16)
|
NCHPSC |
55.13 |
56.32 |
1.19 |
| 7 |
Lennex, Alexander R (16)
|
NCXA |
56.22 |
56.54 |
0.32 |
| 8 |
MacDermott, Maddux D (16)
|
NCRACY |
58.79 |
56.66 |
-2.13 |
| 9 |
Murray, Samuel J (16)
|
NCSTAR |
54.49 |
56.85 |
2.36 |
| 10 |
Williams, Matheson E (15)
|
NCSTAR |
1:00.26 |
59.70 |
-0.56 |
| 11 |
MacDermott, Connor E (15)
|
NCRACY |
1:04.56 |
59.96 |
-4.60 |
| 12 |
Lennex, Landon C (15)
|
NCXA |
1:03.47 |
1:00.88 |
-2.59 |
| 13 |
Stringfield, Robert K (15)
|
NCXA |
1:02.36 |
1:01.71 |
-0.65 |