| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
True, Nathaniel E (12)
|
NCTRY |
33.69 |
33.12 |
-0.57 |
| 2 |
Sawyer, Owen S (12)
|
NCYBAC |
38.90 |
35.25 |
-3.65 |
| 3 |
Nye, Owen R (11)
|
NCYBAC |
34.93 |
35.89 |
0.96 |
| 4 |
Jones, Griffin D (11)
|
NCSFSC |
35.37 |
36.20 |
0.83 |
| 5 |
Williams, Matheson E (12)
|
NCSTAR |
34.44 |
36.66 |
2.22 |
| 6 |
Jacobs, William T (11)
|
NCSFSC |
39.11 |
37.22 |
-1.89 |
| 7 |
Wade, Wesley (11)
|
NCYBAC |
43.88 |
41.19 |
-2.69 |
| 8 |
Ellis, Andrew T (12)
|
NCYBAC |
45.07 |
41.39 |
-3.68 |
| 9 |
Teoh, Reid P (12)
|
NCSTAR |
|
42.44 |
- |
| 10 |
Dodson, Lucas M (12)
|
NCSTAR |
44.92 |
42.67 |
-2.25 |
| 11 |
Nicolet, Foster J (12)
|
NCUN-0 |
44.11 |
43.53 |
-0.58 |
| 12 |
Grant, Aiden P (11)
|
NCYBAC |
43.81 |
43.87 |
0.06 |
| 13 |
Robinette, Griffin D (12)
|
NCSFSC |
|
51.30 |
- |