| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Clara P (9)
|
NCECA |
37.14 |
38.04 |
0.90 |
| 2 |
Ochieng, Gracie A (11)
|
NCECA |
37.27 |
39.99 |
2.72 |
| 3 |
Christian, Allison C (11)
|
NCECA |
43.27 |
40.32 |
-2.95 |
| 4 |
Buskirk, Emmalyn G (11)
|
NCECA |
41.90 |
42.89 |
0.99 |
| 5 |
Francis, Kiarra D (12)
|
NCECA |
44.35 |
45.97 |
1.62 |
| 6 |
Jenkins, Grace J (8)
|
NCECA |
56.42 |
55.11 |
-1.31 |
| 7 |
Gianelloni, Lucy J (8)
|
NCECA |
|
1:07.62 |
- |
| 8 |
Shearer, Isabellah G (9)
|
NCECA |
|
1:09.55 |
- |
| 9 |
Francis, Kamryn J (8)
|
NCECA |
1:04.07 |
1:17.65 |
13.58 |
|
Pendley, Ava E (9)
|
NCECA |
1:03.07 |
NS |
- |
|
Wunderlich, Peyton M (11)
|
NCECA |
34.78 |
NS |
- |