| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Opitz, Lillian K (7)
|
AMPEAQ |
24.28 |
22.70 |
-1.58 |
| 2 |
Turner, Alyssa J (7)
|
AMRACE |
27.18 |
24.59 |
-2.59 |
| 3 |
Murphy, Olivia C (7)
|
AMBWA |
48.16 |
26.87 |
-21.29 |
| 4 |
Alexander, Annabelle E (7)
|
AMSVSC |
34.22 |
27.07 |
-7.15 |
| 5 |
Hollenbeck, Mackenzie E (7)
|
AMANSC |
32.72 |
27.55 |
-5.17 |
| 6 |
Drozd, Emily M (7)
|
AMUN-A |
34.74 |
29.17 |
-5.57 |
| 7 |
Ponder, Alacia M (7)
|
AMRACE |
45.00 |
31.57 |
-13.43 |
| 8 |
Hunsinger, Sadie G (7)
|
AMFAST |
33.31 |
31.80 |
-1.51 |
| 9 |
Proie, Alexandra S (7)
|
AMPRA |
48.57 |
33.83 |
-14.74 |
| 10 |
Bittner, Mia A (7)
|
AMRXA |
45.99 |
34.12 |
-11.87 |
| 11 |
Palkovitch, Scarlet M (7)
|
AMRACE |
41.00 |
41.07 |
0.07 |