| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gorby, Tessa L (12)
|
VAGATR |
26.92 |
27.33 |
0.41 |
| 2 |
Bing, Rachael C (11)
|
VAGATR |
30.04 |
30.06 |
0.02 |
| 3 |
Keller, Madison G (11)
|
VAGATR |
29.21 |
30.07 |
0.86 |
| 4 |
Hooke, Alison P (11)
|
VAGATR |
30.20 |
30.95 |
0.75 |
| 5 |
Yang, Leslie B (11)
|
VAGATR |
35.45 |
32.43 |
-3.02 |
| 6 |
Shaver, Delaney N (12)
|
VAGATR |
32.92 |
34.54 |
1.62 |
| 7 |
Bryant, Blakely (12)
|
VAACST |
35.72 |
34.99 |
-0.73 |
| 8 |
Wiles, Sarah E (12)
|
VABASS |
33.89 |
35.22 |
1.33 |
| 9 |
Wightman, Alice A (12)
|
VAGATR |
35.51 |
35.85 |
0.34 |
| 10 |
Loar, Brook (11)
|
VAACST |
|
36.27 |
- |
| 11 |
Abdulaeva, Nicole (12)
|
VAACST |
38.14 |
37.81 |
-0.33 |
| 12 |
Wohlford, Emma T (11)
|
VAGATR |
37.55 |
38.21 |
0.66 |
| 13 |
Lucius-Milliman, Emma (12)
|
VAACST |
44.24 |
41.85 |
-2.39 |
| 14 |
Gresham, Alice M (11)
|
VASTRM |
42.62 |
43.17 |
0.55 |
|
Sylvester-Johnson, Jillian B (11)
|
VAGATR |
31.31 |
NS |
- |