| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stetor, Wyatt R (10)
|
AMUPEA |
36.58 |
38.46 |
1.88 |
| 2 |
Punturiero, Salvatore A (10)
|
AMPEAQ |
39.24 |
39.76 |
0.52 |
| 3 |
Chen, Alexander B (10)
|
AMJCCS |
41.69 |
41.34 |
-0.35 |
| 4 |
Semenov, Ethan B (10)
|
AMJCCS |
45.37 |
43.38 |
-1.99 |
| 5 |
Singh, Sahil R (10)
|
AMSFAC |
46.14 |
43.72 |
-2.42 |
| 6 |
Lindblad, Matthew N (10)
|
AMANSC |
43.72 |
43.95 |
0.23 |
| 7 |
Lozowski, Nicholas R (10)
|
AMSVSC |
46.44 |
44.49 |
-1.95 |
| 8 |
Pickett, Noah M (10)
|
AMMAC |
46.50 |
48.12 |
1.62 |
| 9 |
Pickett, Christopher B (8)
|
AMMAC |
46.50 |
56.70 |
10.20 |
|
Morrell, Andrew M (10)
|
AMANSC |
42.93 |
NS |
- |
|
Powell, Caden R (10)
|
AMBPR |
43.91 |
NS |
- |