| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bendersky, Hailey R (10)
|
MRWISC |
33.48 |
33.34 |
-0.14 |
| 2 |
DeCasseres, Brianna O (10)
|
MRWEST |
35.58 |
33.35 |
-2.23 |
| 3 |
Behrend, Molly C (10)
|
MRWEST |
35.21 |
33.50 |
-1.71 |
| 4 |
Weinstein, Sophie J (8)
|
MRWEST |
39.78 |
36.88 |
-2.90 |
| 5 |
Sherman, Alexa L (10)
|
MRLIE |
|
37.87 |
- |
| 6 |
McMahon, Reilly E (10)
|
MRWISC |
38.03 |
37.96 |
-0.07 |
| 7 |
Lin, Rong Jia L (10)
|
MRQNS |
37.04 |
38.45 |
1.41 |
| 8 |
Knopf, Cristina R (9)
|
MRSSC |
44.54 |
43.97 |
-0.57 |
| 9 |
Weinstein, Emma J (7)
|
MRWEST |
48.25 |
44.91 |
-3.34 |
| 10 |
Dellagiovanna, Sofia M (10)
|
MRLIE |
|
45.28 |
- |
| 11 |
Kohner, Paige A (7)
|
MRLIE |
|
49.84 |
- |
| 12 |
Hunter, Evelyn L (8)
|
MRLIE |
|
51.22 |
- |
| 13 |
Cokley, Isabelle E (8)
|
MRLIE |
|
53.71 |
- |
| 14 |
Porras, Michaela K (7)
|
MRLIE |
|
1:14.43 |
- |