| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ryland, Claire N (13)
|
MAHAC |
24.80 |
25.11 |
0.31 |
| 2 |
Rippon, Bella M (13)
|
MAHAC |
26.88 |
26.63 |
-0.25 |
| 3 |
Tafuto, Victoria C (13)
|
MAHAC |
26.88 |
26.65 |
-0.23 |
| 4 |
Clow, Jalyn R (13)
|
MAMARA |
28.36 |
29.45 |
1.09 |
| 5 |
Tarkenton, Margaret G (13)
|
MAHAC |
28.81 |
29.56 |
0.75 |
| 6 |
McCormick, Kayla M (14)
|
MAMARA |
30.19 |
29.85 |
-0.34 |
| 7 |
Pedersen, Ella S (14)
|
MAHAC |
30.09 |
29.86 |
-0.23 |
| 8 |
Russell, Anne (14)
|
MAMARA |
27.39 |
29.90 |
2.51 |
| 9 |
Grubb, Kirsten A (14)
|
MAHAC |
31.22 |
31.62 |
0.40 |
| 10 |
Brinkman, Sidney Q (13)
|
MAMARA |
34.55 |
32.20 |
-2.35 |
| 11 |
Fleszar, Laurel V (13)
|
MAHAC |
33.35 |
32.86 |
-0.49 |
| 12 |
Victorino, Mia E (13)
|
MAMARA |
32.88 |
33.32 |
0.44 |
| 13 |
Kauffman, Annalise F (13)
|
MAMARA |
31.89 |
33.49 |
1.60 |
| 14 |
Seminara, Rosemarie V (13)
|
MAMARA |
|
45.00 |
- |
|
Roth, Amanda R (13)
|
MAMARA |
38.39 |
NS |
- |