| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Shea, Charles K (11)
|
NCUN |
39.27 |
38.43 |
-0.84 |
| 2 |
DeCubellis, Elijah T (12)
|
NCMAC |
42.00 |
40.03 |
-1.97 |
| 3 |
Kuck, Sean M (11)
|
NCMAC |
46.36 |
41.32 |
-5.04 |
| 4 |
Anderson, Aidan R (12)
|
NCMAC |
42.31 |
41.82 |
-0.49 |
| 5 |
Bacharach, William J (12)
|
NCYSST |
42.83 |
42.86 |
0.03 |
| 6 |
Tran, Evan V (11)
|
NCMAC |
54.99 |
46.01 |
-8.98 |
| 7 |
Tillman, James C (12)
|
NCNSS |
43.85 |
46.06 |
2.21 |
| 8 |
Gates, William R (11)
|
NCNSS |
45.33 |
46.07 |
0.74 |
| 9 |
Kogan, Maximilian (12)
|
NCMAC |
47.42 |
46.93 |
-0.49 |
| 10 |
Hullette, Oakley T (12)
|
NCYSST |
49.43 |
47.12 |
-2.31 |
| 11 |
Cretu, Nicolae (11)
|
NCWNCY |
|
47.49 |
- |
| 12 |
Slonim, Mikhail B (12)
|
NCMAC |
46.46 |
48.20 |
1.74 |
| 13 |
Bambrick, Ryan S (11)
|
NCNSS |
44.51 |
48.38 |
3.87 |
| 14 |
Nadelman, Stepp M (11)
|
NCNSS |
45.23 |
48.39 |
3.16 |
| 15 |
Jackson, Preston B (11)
|
NCUN |
53.95 |
49.05 |
-4.90 |
| 16 |
Ruskin, Evan M (11)
|
NCMAC |
50.77 |
51.51 |
0.74 |
| 17 |
Cassidy, Carter P (11)
|
NCMAC |
|
51.80 |
- |
| 18 |
Strauss, Garrett B (11)
|
NCWNCY |
53.55 |
55.85 |
2.30 |
| 19 |
Copenhaver, Ryan N (11)
|
NCWNCY |
54.89 |
56.20 |
1.31 |
|
Jackson, Parker T (12)
|
NCUN |
1:05.16 |
DQ |
- |
|
Bell, Jonah T (12)
|
NCWNCY |
58.67 |
NS |
- |