| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thornburg, Emma G (12)
|
NCGG |
32.80 |
31.22 |
-1.58 |
| 2 |
Johnson, Margaret K (12)
|
NCCVAC |
33.84 |
32.43 |
-1.41 |
| 3 |
Sanchez, Aryanna I (11)
|
NCWA |
33.93 |
33.48 |
-0.45 |
| 4 |
Canipe, Libby G (12)
|
NCSAC |
35.12 |
34.19 |
-0.93 |
| 5 |
Rodriquez, Maria V (11)
|
NCMSC |
34.12 |
34.71 |
0.59 |
| 6 |
Olguin-Abreu, Denise (12)
|
NCCVAC |
34.02 |
34.84 |
0.82 |
| 7 |
Beltran-Trejo, Nicole (12)
|
NCCVAC |
34.29 |
35.69 |
1.40 |
| 8 |
Olvera, Karina (12)
|
NCCVAC |
35.37 |
35.71 |
0.34 |
| 9 |
Stewart, Adelee L (11)
|
NCGG |
|
35.91 |
- |
| 10 |
Walters, Abigail R (11)
|
NCCVAC |
35.15 |
36.20 |
1.05 |
| 11 |
Murphy, Riley J (12)
|
NCLENR |
34.16 |
36.31 |
2.15 |
| 12 |
Caudle, Riley S (11)
|
NCCVAC |
38.26 |
38.65 |
0.39 |
| 13 |
Davison, Lillian M (12)
|
NCGG |
38.12 |
38.79 |
0.67 |
| 14 |
Kale, Zelina M (11)
|
NCCVAC |
38.55 |
39.02 |
0.47 |
| 15 |
Yelton, Lucy E (11)
|
NCMSC |
44.75 |
40.25 |
-4.50 |
| 16 |
Barker, Laurel R (11)
|
NCLENR |
44.91 |
42.57 |
-2.34 |
|
Byrd, Legacy (11)
|
NCCVAC |
|
NS |
- |