| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ai, Bethany R (12)
|
NCCVAC |
|
31.57 |
- |
| 2 |
Johnson, Margaret K (12)
|
NCCVAC |
32.43 |
32.16 |
-0.27 |
| 3 |
Driessen, Tarraleah L (12)
|
NCNMA |
33.69 |
33.31 |
-0.38 |
| 4 |
Loranger, Emma K (11)
|
NCNMA |
35.35 |
33.33 |
-2.02 |
| 5 |
Sanchez, Aryanna I (11)
|
NCWA |
33.28 |
33.45 |
0.17 |
| 6 |
Hilderbran, Ella N (12)
|
NCGG |
33.76 |
33.82 |
0.06 |
| 7 |
Olguin-Abreu, Denise (12)
|
NCCVAC |
34.02 |
33.98 |
-0.04 |
| 8 |
Olvera, Karina (12)
|
NCCVAC |
35.37 |
34.19 |
-1.18 |
| 9 |
Lawrence, Amelia S (11)
|
NCWA |
36.68 |
36.37 |
-0.31 |
| 10 |
Caudle, Riley S (12)
|
NCCVAC |
38.26 |
37.92 |
-0.34 |
| 11 |
Hadebe, Wandisile S (12)
|
NCNMA |
39.17 |
38.46 |
-0.71 |
| 12 |
Miller, McNeal R (11)
|
NCGG |
38.87 |
39.00 |
0.13 |
| 13 |
Kale, Zelina M (12)
|
NCCVAC |
38.55 |
39.91 |
1.36 |
| 14 |
Jones, Reilly E (11)
|
NCWA |
39.60 |
40.50 |
0.90 |
| 15 |
Byrd, Legacy F (11)
|
NCCVAC |
43.93 |
42.81 |
-1.12 |
|
Lynch, Kirsten N (12)
|
NCGG |
30.43 |
NS |
- |