| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crisp, Caroline P (11)
|
NCWA |
42.84 |
42.20 |
-0.64 |
| 2 |
Hilderbran, Ella N (12)
|
NCGG |
42.09 |
42.27 |
0.18 |
| 3 |
Johnson, Margaret K (12)
|
NCCVAC |
|
43.27 |
- |
| 4 |
Ai, Bethany R (12)
|
NCCVAC |
|
44.08 |
- |
| 5 |
Lee, Jovie M (11)
|
NCCVAC |
45.27 |
45.54 |
0.27 |
| 6 |
Sanchez, Aryanna I (11)
|
NCWA |
45.67 |
46.07 |
0.40 |
| 7 |
Caudle, Riley S (12)
|
NCCVAC |
48.37 |
46.91 |
-1.46 |
| 8 |
Beltran-Trejo, Nicole (12)
|
NCCVAC |
47.99 |
47.47 |
-0.52 |
| 9 |
Loranger, Emma K (11)
|
NCNMA |
53.46 |
49.39 |
-4.07 |
| 10 |
Olvera, Karina (12)
|
NCCVAC |
51.66 |
49.51 |
-2.15 |
| 11 |
Miller, McNeal R (11)
|
NCGG |
48.46 |
49.53 |
1.07 |
| 12 |
Olguin-Abreu, Denise (12)
|
NCCVAC |
50.04 |
50.25 |
0.21 |
| 13 |
Lawrence, Amelia S (11)
|
NCWA |
49.70 |
53.07 |
3.37 |
| 14 |
Jones, Reilly E (11)
|
NCWA |
48.80 |
53.37 |
4.57 |
| 15 |
Hadebe, Wandisile S (12)
|
NCNMA |
56.42 |
55.73 |
-0.69 |
| 16 |
Byrd, Legacy F (11)
|
NCCVAC |
1:01.86 |
1:03.94 |
2.08 |