| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hollifield, Meredith V (12)
|
NCRCST |
32.81 |
31.97 |
-0.84 |
| 2 |
Thomas, Paige D (12)
|
NCGG |
32.43 |
33.46 |
1.03 |
| 3 |
Hedrick, Chloe M (12)
|
NCCVAC |
35.17 |
33.85 |
-1.32 |
| 4 |
Huffman, Sydney M (11)
|
NCCVAC |
36.70 |
36.82 |
0.12 |
| 5 |
Wentz, Elizabeth R (12)
|
NCWA |
|
38.77 |
- |
| 6 |
Outlaw, Lizzie L (12)
|
NCWA |
41.37 |
39.36 |
-2.01 |
| 7 |
Jones, Merritt M (11)
|
NCRCST |
41.61 |
40.71 |
-0.90 |
| 8 |
Allen, Jessica (11)
|
NCMSC |
|
41.24 |
- |
| 9 |
Clontz, Leah J (12)
|
NCCVAC |
37.97 |
41.31 |
3.34 |
| 10 |
Finch, Fiona A (12)
|
NCCVAC |
|
43.91 |
- |
| 11 |
Carswell, Kennedy G (12)
|
NCMSC |
47.94 |
46.19 |
-1.75 |
| 12 |
Rodriguez, Keyla L (12)
|
NCMSC |
48.61 |
46.89 |
-1.72 |
| 13 |
Schlageter, Jordan A (12)
|
NCLENR |
|
49.35 |
- |
| 14 |
McPeak, Laci K (11)
|
NCLENR |
57.91 |
51.70 |
-6.21 |
| 15 |
Elder, Jenna E (12)
|
NCCVAC |
|
52.65 |
- |
| 16 |
Cullen, Riley W (11)
|
NCMSC |
|
52.76 |
- |
| 17 |
Helderman, Maggie L (12)
|
NCLENR |
|
1:07.07 |
- |
|
Hilderbran, Ella N (11)
|
NCGG |
48.99 |
DQ |
- |
|
Stafford, Makenna L (12)
|
NCCVAC |
|
DQ |
- |
|
Olvera, Karina (11)
|
NCCVAC |
|
DQ |
- |
|
Lindquist, Kathryn M (11)
|
NCGG |
41.80 |
NS |
- |