| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Balbuena, Gabriela C (12)
|
NCWAVE |
33.44 |
33.03 |
-0.41 |
| 2 |
Paleocrassas, Daphne M (11)
|
NCWAVE |
|
37.28 |
- |
| 3 |
McWhorter, Kylie A (11)
|
SCMBR |
38.08 |
37.86 |
-0.22 |
| 4 |
Paleocrassas, Katerina E (12)
|
NCWAVE |
|
38.32 |
- |
| 5 |
Speir, Ella N (11)
|
SCMBR |
37.04 |
38.73 |
1.69 |
| 6 |
Hall, Zion D (11)
|
NCWAVE |
42.91 |
39.15 |
-3.76 |
| 7 |
Habib, Ella R (11)
|
SCMBR |
38.32 |
39.43 |
1.11 |
| 8 |
Rose, Ireland K (11)
|
SCMBR |
40.32 |
39.84 |
-0.48 |
| 9 |
Rouser, Kennedy R (12)
|
NCWAVE |
40.97 |
40.24 |
-0.73 |
| 10 |
Sarmiere, Alexa P (11)
|
NCWAVE |
45.05 |
41.98 |
-3.07 |
| 11 |
Ouellette, Mia G (11)
|
NCGFSC |
53.49 |
52.94 |
-0.55 |
| 12 |
Brown, Miller G (11)
|
NCWAVE |
54.22 |
53.65 |
-0.57 |
| 13 |
Aghoram, Aditi (11)
|
NCWAVE |
1:05.05 |
1:05.32 |
0.27 |