| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wesley, Anna C (8)
|
NCACCH |
53.64 |
46.30 |
-7.34 |
| 2 |
Brewer, Rylee G (8)
|
NCRMY |
51.50 |
46.75 |
-4.75 |
| 3 |
Yorkery, Cadence A (8)
|
NCNSS |
51.50 |
47.65 |
-3.85 |
| 4 |
Milligan, Sarah M (7)
|
NCRMY |
48.85 |
49.03 |
0.18 |
| 5 |
Overby, Kayla A (7)
|
NCHAC |
52.34 |
49.43 |
-2.91 |
| 6 |
Johnson, Emory R (8)
|
NCFAST |
49.21 |
49.95 |
0.74 |
| 7 |
Challa, Maya I (8)
|
NCHAC |
50.61 |
50.47 |
-0.14 |
| 8 |
Pattison, Kathryn E (8)
|
NCHAC |
|
52.45 |
- |
| 9 |
Brown, Jasmin S (7)
|
NCHAC |
|
1:11.54 |
- |
| 10 |
Stephens, Emma W (8)
|
NCACCH |
|
1:17.09 |
- |
|
Grau, Taylor M (7)
|
NCNSS |
|
DQ |
- |
|
Herlocker, Patterson L (6)
|
NCRACY |
|
DQ |
- |