| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dewalt, Kieran M (15)
|
NCACCH |
52.10 |
50.65 |
-1.45 |
| 2 |
McCarthy, Zachary B (18)
|
NCYOTA |
51.10 |
50.90 |
-0.20 |
| 3 |
Cain, Jackson C (16)
|
NCYOTA |
51.43 |
50.99 |
-0.44 |
| 4 |
Ward, Ian M (17)
|
NCACCH |
51.22 |
51.36 |
0.14 |
| 5 |
Williams, Camden H (15)
|
NCWA |
54.16 |
51.69 |
-2.47 |
| 6 |
McGovern, Camden (15)
|
NCYOTA |
50.02 |
51.80 |
1.78 |
| 7 |
Day, Collin M (16)
|
NCYOTA |
53.57 |
53.24 |
-0.33 |
| 8 |
Humbert, William S (18)
|
NCYOTA |
50.67 |
53.35 |
2.68 |
| 9 |
Roman, Cross M (16)
|
NCHAC |
53.89 |
53.77 |
-0.12 |
| 10 |
Jeffries, Andrew L (17)
|
NCYOTA |
53.79 |
53.82 |
0.03 |
| 11 |
Klein, Jonathan E (61)
|
NCHAC |
55.59 |
54.59 |
-1.00 |
| 12 |
Anderson, Nicholas D (15)
|
NCYOTA |
53.96 |
54.90 |
0.94 |
| 13 |
Walters, Antonio L (18)
|
NCYBAC |
54.44 |
55.24 |
0.80 |
| 14 |
Doughty, John J (17)
|
NCYOTA |
56.26 |
55.61 |
-0.65 |
| 15 |
Nowacek, George A (15)
|
NCACCH |
59.76 |
58.76 |
-1.00 |
| 16 |
Harris, Theodore L (15)
|
NCACCH |
1:01.90 |
1:01.83 |
-0.07 |
| 17 |
Teague, Jeffery B (18)
|
NCWA |
59.24 |
1:02.29 |
3.05 |
| 18 |
Brouwer, Ethan G (15)
|
NCYBAC |
1:04.79 |
1:02.69 |
-2.10 |
| 19 |
Johnson, Christen E (15)
|
NCHAC |
1:05.53 |
1:06.80 |
1.27 |
|
Clippinger, Andrew T (16)
|
NCYOTA |
1:02.95 |
NS |
- |
|
Cotton, Michael J (16)
|
NCACCH |
53.20 |
NS |
- |