| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fu, Jiayu (16)
|
NCWAVE |
26.04 |
25.45 |
-0.59 |
| 2 |
Adams, Jennifer A (15)
|
NCWAVE |
24.85 |
26.42 |
1.57 |
| 3 |
Acome, Brianne P (15)
|
NCHAC |
25.74 |
26.44 |
0.70 |
| 4 |
Erickson, Grace A (16)
|
NCHAC |
28.28 |
26.61 |
-1.67 |
| 5 |
Sudderth, Marissa G (15)
|
NCHAC |
26.01 |
26.74 |
0.73 |
| 6 |
Hunsberger, Eliot L (16)
|
NCHAC |
26.34 |
26.91 |
0.57 |
| 7 |
Baldwin, LeighAnne N (17)
|
NCWAVE |
26.41 |
26.92 |
0.51 |
| 7 |
Galloway, Claire E (17)
|
NCWAVE |
26.29 |
26.92 |
0.63 |
| 9 |
Kennedy, Carlyle R (15)
|
NCWAVE |
25.22 |
27.07 |
1.85 |
| 10 |
Petschauer, Madison A (16)
|
NCWAVE |
26.75 |
27.17 |
0.42 |
| 11 |
Gooch, Ellen J (18)
|
NCHAC |
27.14 |
27.46 |
0.32 |
| 12 |
Edelson, Anna E (17)
|
NCWAVE |
26.07 |
27.55 |
1.48 |
| 13 |
Fullerton, Mikaela C (17)
|
NCWAVE |
25.46 |
27.59 |
2.13 |
| 14 |
Hurlbert, Katherine R (17)
|
NCWAVE |
25.83 |
27.94 |
2.11 |
| 15 |
Edelson, Elena F (15)
|
NCWAVE |
27.73 |
29.42 |
1.69 |
|
Gschwind, Olivia C (16)
|
NCWAVE |
24.65 |
DQ |
- |