| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ingraham, Leah M (14)
|
NIGRSC |
5:10.70 |
5:17.27 |
6.57 |
| 2 |
Reitz, Meghan E (17)
|
NIHAST |
5:12.89 |
5:24.03 |
11.14 |
| 3 |
Shearing, McKenna E (13)
|
NIPACK |
6:10.60 |
5:30.49 |
-40.11 |
| 4 |
Doughty, Emma J (15)
|
NIHAST |
5:31.00 |
5:31.05 |
0.05 |
| 5 |
Stepanek, Livia G (14)
|
NIHAST |
5:47.50 |
5:31.15 |
-16.35 |
| 6 |
Bromage, Charlotte C (13)
|
NIQUIK |
5:56.00 |
5:33.74 |
-22.26 |
| 7 |
Kennedy, Madaliene F (14)
|
NISDSC |
5:55.26 |
5:34.32 |
-20.94 |
| 8 |
Evans, Mary A (12)
|
NIGRSC |
5:38.32 |
5:43.06 |
4.74 |
| 9 |
Geoca, Zoey L (14)
|
NISDSC |
5:39.10 |
5:43.32 |
4.22 |
| 10 |
Sahin, Melis (13)
|
NIPACK |
5:46.96 |
5:54.99 |
8.03 |
| 11 |
Perrah, Victoria R (16)
|
NIGRSC |
|
6:01.13 |
- |
| 12 |
Christodaro, Melina A (15)
|
NIHAST |
6:06.03 |
6:01.24 |
-4.79 |
| 13 |
Zona, Gianna I (11)
|
NIGRSC |
|
6:34.68 |
- |
| 14 |
Newsome, Amber G (11)
|
NIPMSC |
|
6:59.01 |
- |
| 15 |
Leslie, Jordyn A (14)
|
NIHAST |
7:12.50 |
7:02.77 |
-9.73 |