| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reitz, Meghan E (18)
|
NIHAST |
5:12.89 |
5:16.27 |
3.38 |
| 2 |
Brennan, Molly (16)
|
NIPACK |
4:42.38 |
5:17.30 |
34.92 |
| 3 |
Stepanek, Livia G (15)
|
NIHAST |
5:23.93 |
5:21.81 |
-2.12 |
| 4 |
Miller, Alayna E (13)
|
NIHAST |
5:46.31 |
5:49.32 |
3.01 |
| 5 |
Bonfiglio, Sara F (16)
|
NIGRSC |
5:49.14 |
5:49.34 |
0.20 |
| 6 |
Christodaro, Melina A (16)
|
NIHAST |
6:01.24 |
5:54.19 |
-7.05 |
| 7 |
Sangasy, KhaNy K (12)
|
NIUN-H |
5:45.10 |
5:55.22 |
10.12 |
| 8 |
Amering, Sophie M (13)
|
NIHAST |
6:08.83 |
5:59.16 |
-9.67 |
| 9 |
Bouwens, Emma L (11)
|
NIHAST |
6:38.41 |
6:00.43 |
-37.98 |
| 10 |
Smolinski, Evelynn J (11)
|
NIHAST |
|
6:23.73 |
- |
| 11 |
Quigley, Brienne K (13)
|
NIGRSC |
|
6:43.61 |
- |
| 12 |
Christodaro, Raena M (13)
|
NIHAST |
|
6:45.67 |
- |