| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rottink, Roos A (9)
|
NIPACK |
|
2:59.35 |
- |
| 2 |
Sahin, Hazal (10)
|
NIPACK |
3:16.17 |
3:00.75 |
-15.42 |
| 3 |
Bouwens, Emma L (10)
|
NIHAST |
3:10.36 |
3:11.57 |
1.21 |
| 4 |
Share, Elizabeth A (10)
|
NIPMSC |
3:20.69 |
3:12.18 |
-8.51 |
| 5 |
Knausz, Eleanor M (9)
|
NISDSC |
3:15.08 |
3:17.24 |
2.16 |
| 6 |
Resch, Rebecka J (10)
|
NIGRSC |
3:28.53 |
3:28.75 |
0.22 |
| 7 |
Cafarelli, Brianna N (9)
|
NIQUIK |
|
3:30.27 |
- |
| 8 |
Robinson, Sara C (10)
|
NISDSC |
|
3:35.33 |
- |
| 9 |
Anthony, Jillian M (9)
|
NIPMSC |
3:51.76 |
3:46.86 |
-4.90 |
| 10 |
Chen, Chloe Y (9)
|
NISDSC |
4:13.67 |
3:52.87 |
-20.80 |
| 11 |
Man, Shaila T (10)
|
NIPACK |
|
4:12.76 |
- |
| 12 |
Rist, Olivia B (10)
|
NIHAST |
|
4:18.65 |
- |
|
Smolinski, Evelynn J (10)
|
NIHAST |
|
DQ |
- |
|
Greisch, Alisha D (10)
|
NIGRSC |
4:02.00 |
NS |
- |
|
Siciliano, Paige E (10)
|
NIGRSC |
3:27.86 |
NS |
- |