| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Evans, Mary A (12)
|
NIGRSC |
2:39.66 |
2:35.13 |
-4.53 |
| 2 |
Caldwell, Hannah M (11)
|
NIUN-V |
2:49.52 |
2:45.78 |
-3.74 |
| 3 |
Sanson, Charlotte E (12)
|
NIPMSC |
2:52.90 |
2:46.00 |
-6.90 |
| 4 |
Natemeier, Amelia E (11)
|
NIUN-V |
2:57.33 |
2:52.50 |
-4.83 |
| 5 |
Flanigen, Ruby J (11)
|
NIIRON |
2:56.97 |
2:54.01 |
-2.96 |
| 6 |
Robinson, Sara C (11)
|
NISDSC |
3:07.36 |
3:01.45 |
-5.91 |
| 7 |
Bouwens, Emma L (11)
|
NIHAST |
3:10.36 |
3:02.52 |
-7.84 |
| 8 |
Smolinski, Evelynn J (11)
|
NIHAST |
|
3:10.95 |
- |
| 9 |
LaChance, Carmindy (11)
|
NIIRON |
3:17.57 |
3:21.27 |
3.70 |
| 10 |
Kuhlkin, Elizabeth C (11)
|
NIGCOM |
|
3:24.42 |
- |
| 11 |
Olbrys, Lilja (11)
|
NIVICT |
3:35.41 |
3:29.16 |
-6.25 |
| 12 |
Osborne, Samantha R (12)
|
NIGRSC |
4:01.14 |
3:39.74 |
-21.40 |
|
Johnson, Ila A (12)
|
NIIRON |
3:15.00 |
DQ |
- |