| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Tyler N (16)
|
NIUN-V |
22.86 |
23.45 |
0.59 |
| 2 |
Regna, Alex J (17)
|
NISEAS |
23.35 |
23.79 |
0.44 |
| 3 |
Dambra, Matthew J (17)
|
NISEAS |
23.77 |
23.93 |
0.16 |
| 4 |
Thomas, Joshua D (15)
|
NIOLSC |
24.71 |
24.41 |
-0.30 |
| 5 |
Murphy, Brayden (14)
|
NIVICT |
24.50 |
24.90 |
0.40 |
| 6 |
Frear, Garrett B (17)
|
NIIRON |
25.80 |
25.62 |
-0.18 |
| 7 |
Zani, Samuel A (14)
|
NIIRON |
26.46 |
25.85 |
-0.61 |
| 8 |
DiBitetto, Jack J (14)
|
NIHAST |
25.22 |
25.94 |
0.72 |
| 9 |
Dambra, Nicholas (14)
|
NISEAS |
28.00 |
25.97 |
-2.03 |
| 10 |
Donovan, Rylan (16)
|
NISEAS |
|
25.99 |
- |
| 11 |
Wihlen, Christopher M (14)
|
NIVICT |
26.45 |
26.10 |
-0.35 |
| 11 |
Porretta, Gerald A (13)
|
NIIRON |
26.11 |
26.10 |
-0.01 |
| 13 |
Coyle, Samuel J (14)
|
NISEAS |
26.28 |
26.17 |
-0.11 |
| 14 |
Riexinger, Colby J (14)
|
NISEAS |
|
26.21 |
- |
| 15 |
Smith, Connor J (13)
|
NIUN-V |
26.62 |
26.50 |
-0.12 |
| 16 |
Godown, Elijah M (15)
|
NIPMSC |
25.92 |
26.94 |
1.02 |
| 16 |
Schultz, Colin J (15)
|
NIHAST |
26.82 |
26.94 |
0.12 |
| 18 |
Enser, Jared M (13)
|
NIHAST |
27.67 |
27.11 |
-0.56 |
| 19 |
Murray, Maxwell P (14)
|
NISDSC |
26.03 |
27.74 |
1.71 |
| 20 |
Morgan, Phillip A (14)
|
NIMERC |
|
28.12 |
- |
| 21 |
Michalowski, Connor J (14)
|
NIHAST |
28.96 |
28.16 |
-0.80 |
| 22 |
DeMartinis, Ethan W (13)
|
NIMERC |
28.36 |
28.33 |
-0.03 |
| 23 |
DiFante, Mitchell T (13)
|
NISEAS |
28.90 |
29.25 |
0.35 |
| 24 |
Mueller, Christopher J (14)
|
NISDSC |
30.41 |
29.63 |
-0.78 |
| 25 |
Cunningham, Dalton M (13)
|
NISEAS |
30.14 |
29.71 |
-0.43 |
| 26 |
McCarthy, John E (10)
|
NISDSC |
|
39.38 |
- |
| 27 |
Pompeii, Joey J (13)
|
NIPMSC |
39.59 |
39.46 |
-0.13 |
| 28 |
Newsome, George W (9)
|
NIPMSC |
49.67 |
48.85 |
-0.82 |
|
Derousie, Mitchell B (14)
|
NIHAST |
30.45 |
NS |
- |