| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Larue, Jessie A (16)
|
NIUN-M |
|
27.63 |
- |
| 2 |
DiFante, Marlayna R (16)
|
NIGCOM |
27.48 |
28.58 |
1.10 |
| 3 |
Jackel, Summer N (16)
|
NIGRSC |
28.13 |
28.71 |
0.58 |
| 4 |
Salonen, Amber (15)
|
NIGCOM |
28.98 |
29.64 |
0.66 |
| 5 |
Amering, Sophie M (13)
|
NIHAST |
31.94 |
29.67 |
-2.27 |
| 6 |
Wallace, Emma E (14)
|
NIGRAT |
29.25 |
29.81 |
0.56 |
| 7 |
Salonen-Roperti, Pearl M (14)
|
NIGCOM |
29.02 |
29.92 |
0.90 |
| 8 |
Grego, Liliya E (14)
|
NIGCOM |
30.33 |
29.94 |
-0.39 |
| 9 |
Miller, Anna M (13)
|
NIGRSC |
31.09 |
30.06 |
-1.03 |
| 10 |
Robertson, Julia E (14)
|
NIRRST |
32.55 |
30.18 |
-2.37 |
| 11 |
Coyle, Muriel I (13)
|
NIGCOM |
31.09 |
30.29 |
-0.80 |
| 12 |
Galusha, Alexis P (13)
|
NIGRSC |
31.67 |
30.62 |
-1.05 |
| 13 |
Harris, Saiya'Kaede Z (13)
|
NIRRST |
31.48 |
30.95 |
-0.53 |
| 14 |
Camman, Kylie L (14)
|
NIGRSC |
34.09 |
32.89 |
-1.20 |
| 15 |
Christodaro, Raena M (13)
|
NIHAST |
33.21 |
33.07 |
-0.14 |
| 16 |
Salonen-Roperti, Marissa A (16)
|
NIGCOM |
30.07 |
33.40 |
3.33 |
| 17 |
Morris, Emlyn O (12)
|
NIPACK |
34.80 |
33.72 |
-1.08 |
| 18 |
Tornow, Emilee N (15)
|
NISDSC |
33.84 |
34.30 |
0.46 |
| 19 |
Thompson, Daniella J (13)
|
NIGRSC |
38.67 |
37.37 |
-1.30 |
| 20 |
Bolte-Pauley, Ashlyn R (14)
|
NIGRAT |
|
39.42 |
- |
|
Brennan, Sarah D (16)
|
NIGRSC |
30.03 |
NS |
- |
|
Williams, Tory L (13)
|
NIPACK |
30.61 |
NS |
- |