| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DiBitetto, Jack J (15)
|
NIHAST |
25.22 |
25.78 |
0.56 |
| 2 |
Dinh, Benson (13)
|
NIGRSC |
26.46 |
26.09 |
-0.37 |
| 3 |
Oneil, Callum (14)
|
NIPACK |
27.80 |
26.40 |
-1.40 |
| 4 |
Enser, Jared M (13)
|
NIHAST |
27.11 |
26.62 |
-0.49 |
| 5 |
Johnston, Nicholas W (14)
|
NIPACK |
27.63 |
27.53 |
-0.10 |
| 6 |
Michalowski, Connor J (14)
|
NIHAST |
28.16 |
28.30 |
0.14 |
| 7 |
Gillis, Ian T (15)
|
NIGRSC |
31.00 |
28.94 |
-2.06 |
| 8 |
Wolcott, Charles T (13)
|
NIGRSC |
29.99 |
29.73 |
-0.26 |
| 9 |
Derousie, Mitchell B (15)
|
NIHAST |
30.45 |
29.75 |
-0.70 |
| 10 |
Ciravola, Jayden T (14)
|
NIHAST |
31.80 |
30.83 |
-0.97 |
| 11 |
Fan, Timothy (10)
|
NIPACK |
32.47 |
32.97 |
0.50 |
| 12 |
Green, William E (12)
|
NIHAST |
35.67 |
35.52 |
-0.15 |
| 13 |
Pompeii, Joey J (13)
|
NIPMSC |
39.35 |
35.54 |
-3.81 |
| 14 |
Wooden, Carter J (11)
|
NIUN-Q |
35.42 |
38.64 |
3.22 |
| 15 |
Green, Trent M (10)
|
NIHAST |
38.07 |
39.64 |
1.57 |
|
Goltsman, Joshua A (14)
|
NIPACK |
28.73 |
NS |
- |