| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Enser, Jared M (14)
|
NIHAST |
24.79 |
23.81 |
-0.98 |
| 2 |
Labue, Payton M (15)
|
NIHAST |
24.94 |
24.31 |
-0.63 |
| 3 |
Delehanty, Aidan W (14)
|
NIRRST |
24.01 |
24.45 |
0.44 |
| 4 |
Morreale, Alexander H (14)
|
NIHAST |
25.20 |
24.92 |
-0.28 |
| 5 |
Scaglione, Alexander M (16)
|
NIGCOM |
24.97 |
24.99 |
0.02 |
| 6 |
DiBitetto, Jack J (16)
|
NIHAST |
24.95 |
25.06 |
0.11 |
| 7 |
Morgan, Phillip A (15)
|
NIMERC |
24.86 |
25.33 |
0.47 |
| 8 |
Podgers, Thomas J (17)
|
NIHAST |
|
25.84 |
- |
| 9 |
Cottrell, Samuel R (19)
|
NIGRAT |
25.65 |
26.03 |
0.38 |
| 10 |
Michalowski, Connor J (15)
|
NIHAST |
27.77 |
26.93 |
-0.84 |
| 11 |
Cunningham, Dalton M (14)
|
NIGCOM |
27.30 |
27.26 |
-0.04 |
| 12 |
DiFante, Mitchell T (14)
|
NIGCOM |
27.91 |
27.93 |
0.02 |
| 13 |
Derousie, Mitchell B (16)
|
NIHAST |
29.75 |
27.99 |
-1.76 |
| 14 |
Fixsen, Jordan J (14)
|
NISDSC |
29.70 |
28.92 |
-0.78 |
| 15 |
Green, William E (13)
|
NIHAST |
34.54 |
34.23 |
-0.31 |
| 16 |
Gargano, Adam L (13)
|
NIHAST |
38.36 |
37.01 |
-1.35 |
|
Morreale, Aidan J (12)
|
NIHAST |
32.42 |
NS |
- |
|
Murphy, Liam (15)
|
NIPACK |
23.52 |
NS |
- |