| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Delehanty, Aidan W (14)
|
NIRRST |
52.89 |
53.30 |
0.41 |
| 2 |
Morreale, Alexander H (14)
|
NIHAST |
55.40 |
54.23 |
-1.17 |
| 3 |
Dinh, Benson (14)
|
NIGRSC |
53.73 |
54.87 |
1.14 |
| 4 |
DiBitetto, Jack J (16)
|
NIHAST |
55.99 |
55.98 |
-0.01 |
| 5 |
Scaglione, Alexander M (16)
|
NIGCOM |
56.72 |
57.42 |
0.70 |
| 6 |
Michalowski, Connor J (15)
|
NIHAST |
1:00.15 |
58.16 |
-1.99 |
| 7 |
Cottrell, Samuel R (19)
|
NIGRAT |
57.54 |
59.26 |
1.72 |
| 8 |
DiFante, Mitchell T (14)
|
NIGCOM |
1:01.80 |
1:01.10 |
-0.70 |
| 9 |
Gavigan, Seamus E (12)
|
NIHAST |
1:28.61 |
1:18.97 |
-9.64 |
| 10 |
Green, William E (13)
|
NIHAST |
1:18.59 |
1:19.33 |
0.74 |
| 11 |
Gargano, Adam L (13)
|
NIHAST |
1:35.66 |
1:24.82 |
-10.84 |
| 12 |
Edgecombe, Chase M (11)
|
NIHAST |
1:42.90 |
1:37.89 |
-5.01 |
|
Morreale, Aidan J (12)
|
NIHAST |
1:17.10 |
NS |
- |