| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ewing, Kennedy P (8)
|
NIGVST |
23.07 |
22.27 |
-0.80 |
| 2 |
Leonard, Kaitllyn S (8)
|
NIWAAC |
26.34 |
22.86 |
-3.48 |
| 3 |
Blakely-Armitage, Fiona S (8)
|
NIISWM |
28.36 |
23.57 |
-4.79 |
| 4 |
Ryan, Natalie C (8)
|
NIUNAC |
|
25.38 |
- |
| 5 |
Metz, Isabel L (8)
|
NIKVAC |
|
25.65 |
- |
| 6 |
Homer, Olivia K (7)
|
NIWAAC |
30.92 |
26.82 |
-4.10 |
| 7 |
Tyler, Natalie L (7)
|
NIUN |
|
27.33 |
- |
| 8 |
Clifford, Mikaia M (8)
|
NICORY |
25.24 |
27.35 |
2.11 |
| 9 |
Chandler, Nora M (7)
|
NICVSC |
27.38 |
28.20 |
0.82 |
| 10 |
Cafferty, Myla M (8)
|
NICVSC |
28.60 |
29.35 |
0.75 |
| 11 |
Gonzalez, Leah K (7)
|
NIKRKN |
|
32.01 |
- |
| 12 |
Erb, Makayla A (7)
|
NIUNAC |
|
32.93 |
- |
| 13 |
Spencer, Danielle H (6)
|
NIWAAC |
37.03 |
36.31 |
-0.72 |
| 14 |
Fitzwater, Elizabeth O (7)
|
NIGGST |
|
39.06 |
- |
| 15 |
Howey, Grace A (6)
|
NIWAAC |
|
55.07 |
- |