| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Salazar, Santiago (12)
|
GUPFL |
33.59 |
30.30 |
-3.29 |
| 2 |
Pack, Taylor L (12)
|
GUESA |
34.39 |
32.51 |
-1.88 |
| 3 |
Truan, Charlie G (12)
|
GULIFE |
34.47 |
32.71 |
-1.76 |
| 4 |
Ruppert, Grayson E (11)
|
GUPFL |
34.92 |
32.86 |
-2.06 |
| 5 |
Smith, Parker E (11)
|
GULSST |
33.76 |
32.92 |
-0.84 |
| 6 |
Puecher, Alan (12)
|
GUPFL |
36.84 |
33.15 |
-3.69 |
| 7 |
Nendza, Benjamin M (11)
|
GUSCAT |
33.98 |
33.72 |
-0.26 |
| 8 |
Sager, Caleb M (12)
|
GULIFE |
35.90 |
34.28 |
-1.62 |
| 9 |
Arrambide, Octavio (12)
|
GUESA |
36.90 |
34.86 |
-2.04 |
| 10 |
Lundquist, Rune A (12)
|
GUSWIM |
34.83 |
35.12 |
0.29 |
| 11 |
Sun, Nate A (12)
|
GUPFL |
48.62 |
35.35 |
-13.27 |
| 12 |
Brewer, Zachary B (11)
|
GUSWIM |
38.12 |
35.74 |
-2.38 |
| 13 |
Smeltzer, Luke M (11)
|
GUESA |
35.75 |
35.90 |
0.15 |
| 14 |
Singh, Jaiveer N (12)
|
GUSWIM |
36.14 |
36.32 |
0.18 |
| 15 |
Abad, Ryland D (11)
|
GUSWIM |
38.55 |
36.48 |
-2.07 |
| 16 |
Burns, Brody D (11)
|
GUNEHA |
|
36.58 |
- |
| 17 |
Foltz, Casey J (11)
|
GUESA |
38.17 |
36.66 |
-1.51 |
| 18 |
Djangmah, Nathan J (12)
|
GUTYES |
36.12 |
36.68 |
0.56 |
| 19 |
Blacklock, Ian A (12)
|
GUUNAT |
39.01 |
36.75 |
-2.26 |
| 20 |
Doan, Dawson A (12)
|
GULSST |
37.65 |
37.19 |
-0.46 |
| 21 |
Jistel, Caleb M (12)
|
GUTYES |
38.21 |
37.23 |
-0.98 |
| 22 |
Barrett, Harrison L (12)
|
GULSST |
39.39 |
37.30 |
-2.09 |
| 23 |
Cornelius, Kelten B (12)
|
GUSST |
|
37.55 |
- |
| 24 |
Avalos, Mario A (12)
|
GUFRST |
|
37.58 |
- |
| 25 |
Nash, Mason J (12)
|
GUSWIM |
37.84 |
37.97 |
0.13 |
| 26 |
Reitmeyer, William F (12)
|
GUNEHA |
40.68 |
38.72 |
-1.96 |
| 27 |
Smith, Owen J (11)
|
GUESA |
41.14 |
38.79 |
-2.35 |
| 28 |
Alford, Colson B (11)
|
GUSWIM |
|
39.10 |
- |
| 29 |
Samonte, Cayman T (12)
|
GULSST |
41.21 |
40.00 |
-1.21 |
| 30 |
Tanig, Reece C (11)
|
GULIFE |
44.20 |
40.58 |
-3.62 |
| 31 |
Cespedes, Adrian J (12)
|
GUBATS |
|
41.69 |
- |
| 32 |
Hicks, William H (11)
|
GUSWIM |
42.55 |
41.86 |
-0.69 |
| 33 |
Obando, Christian A (11)
|
GUUSA |
50.08 |
42.26 |
-7.82 |
| 34 |
Wang, Eric (11)
|
GUBATS |
47.59 |
42.36 |
-5.23 |
| 35 |
King, Denys A (11)
|
GUSCAT |
41.87 |
42.82 |
0.95 |
| 36 |
Brown, Landon D (11)
|
GUESA |
44.10 |
43.00 |
-1.10 |
| 37 |
Rodriguez, Jacob A (12)
|
GUBATS |
45.21 |
43.22 |
-1.99 |
| 38 |
Lewis, Robert L (11)
|
GUGIST |
|
43.65 |
- |
| 39 |
Nowotny, Grant W (11)
|
GUFRST |
44.49 |
45.09 |
0.60 |
| 40 |
McCallister, Maddox S (12)
|
GUTYES |
44.87 |
45.96 |
1.09 |
| 41 |
Fioca, Stefano (11)
|
GULIFE |
50.69 |
47.72 |
-2.97 |
| 42 |
Frisch, Ryan C (11)
|
GUSWIM |
52.00 |
48.81 |
-3.19 |
|
Flathouse, Colton A (11)
|
GUFRST |
38.66 |
DQ |
- |
|
Mao, Dylan C (11)
|
GUSWIM |
48.88 |
NS |
- |
|
Martinez, Eric A (12)
|
GUSWIM |
37.57 |
NS |
- |
|
Gulley, Zachary J (12)
|
GUBATS |
38.14 |
NS |
- |