| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Garrison, Sophia J (17)
|
STATAC |
27.01 |
25.20 |
-1.81 |
| 2 |
Eckhart, Keilah E (17)
|
GUCFSC |
26.82 |
25.87 |
-0.95 |
| 3 |
Casten, Genevieve C (15)
|
GUCFSC |
26.55 |
26.36 |
-0.19 |
| 4 |
Cates, Tyler K (17)
|
STATAC |
29.04 |
28.16 |
-0.88 |
| 5 |
Barley, Ariel B (16)
|
STATAC |
30.28 |
28.58 |
-1.70 |
| 6 |
Weeks, Sonya C (15)
|
STATAC |
29.08 |
29.01 |
-0.07 |
| 7 |
Yates, Imanee S (16)
|
GUKATY |
30.14 |
29.44 |
-0.70 |
| 8 |
Mahan, Elizabeth J (16)
|
GUKATY |
31.27 |
29.65 |
-1.62 |
| 9 |
Bose, Ariana M (18)
|
STATAC |
29.30 |
29.66 |
0.36 |
| 10 |
Zimmerman, Kathryn D (17)
|
GUCFSC |
32.87 |
29.71 |
-3.16 |
| 11 |
Roberson, Katherine A (18)
|
GUKATY |
29.33 |
30.48 |
1.15 |
| 12 |
Schlather, Sabrina S (17)
|
GUBATS |
30.20 |
30.84 |
0.64 |
| 13 |
Blake, Kylie M (15)
|
GUCFSC |
|
30.87 |
- |
| 14 |
Norris, Grace L (15)
|
GUBATS |
31.63 |
31.53 |
-0.10 |
| 15 |
Budsberg, Addison E (16)
|
GUTWST |
35.69 |
33.01 |
-2.68 |
|
Pulkkinen, Josephine O (15)
|
GUKATY |
34.51 |
NS |
- |
|
Bjelland, Alva (16)
|
GUKATY |
27.79 |
NS |
- |
|
Barton, Samantha V (16)
|
GUUN-K |
|
NS |
- |