| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hearon, Avery L (10)
|
GUAQUA |
2:43.57 |
2:40.91 |
-2.66 |
| 2 |
Caston, Kelsie M (10)
|
GUESA |
2:43.94 |
2:43.22 |
-0.72 |
| 3 |
Smeltzer, Ella R (10)
|
GUESA |
2:56.05 |
2:48.70 |
-7.35 |
| 4 |
Bockhorn, Hayley J (10)
|
GUBTA |
2:49.20 |
2:49.57 |
0.37 |
| 5 |
Hadaegh, Kimiya L (10)
|
GUAQUA |
3:08.56 |
2:57.86 |
-10.70 |
| 6 |
McEvoy, Charlee F (10)
|
GUSHAC |
2:53.93 |
2:58.02 |
4.09 |
| 7 |
Sosa, Lylah E (9)
|
GUBATS |
2:56.11 |
2:58.49 |
2.38 |
| 8 |
Collins, Cali M (10)
|
GUBTA |
2:54.06 |
3:02.33 |
8.27 |
| 8 |
Etter, Morgan E (10)
|
GUBTA |
3:05.76 |
3:02.33 |
-3.43 |
| 10 |
Schauss, Ingrid A (9)
|
GUAQUA |
3:10.09 |
3:03.39 |
-6.70 |
| 11 |
Trevino, Patricia C (10)
|
GUNOCH |
3:11.49 |
3:07.06 |
-4.43 |
| 12 |
Zhu, Jingyi J (10)
|
GUGIST |
3:07.34 |
3:08.26 |
0.92 |
| 13 |
McGonagle, Brielle (10)
|
GUSSS |
3:21.10 |
3:09.38 |
-11.72 |
| 14 |
Sisung, Lilly L (10)
|
GUAQUA |
3:41.48 |
3:36.42 |
-5.06 |
|
San Miguel, Eliana G (10)
|
GUESA |
|
NS |
- |
|
Pekar, Paige O (9)
|
GUSSS |
3:56.93 |
NS |
- |