| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rains, Riley A (13)
|
GUPACK |
3:09.86 |
2:56.86 |
-13.00 |
| 2 |
Shankar, Samita S (13)
|
GUAGS |
2:53.20 |
2:58.71 |
5.51 |
| 3 |
Whitten, Margaret L (13)
|
GUAGS |
3:08.31 |
3:00.48 |
-7.83 |
| 4 |
Grzyb, Kalle E (13)
|
GUPACK |
3:21.18 |
3:12.31 |
-8.87 |
| 5 |
Duan, Ally M (13)
|
GUAGS |
3:09.56 |
3:13.33 |
3.77 |
| 6 |
O'Sullivan, Ciara A (13)
|
GUPACK |
3:18.52 |
3:19.66 |
1.14 |
| 7 |
Pham, Kaley V (14)
|
GUPACK |
3:22.05 |
3:20.10 |
-1.95 |
| 8 |
Martin, Caroline M (14)
|
GUSPA |
3:25.08 |
3:24.40 |
-0.68 |
| 9 |
Morais, Sofia G (13)
|
GUAGS |
|
3:25.36 |
- |
| 10 |
Demlow, Lydia N (14)
|
GUAGS |
3:35.67 |
3:27.51 |
-8.16 |
| 11 |
Spencer, Alexandra J (14)
|
GUPACK |
3:29.64 |
3:28.28 |
-1.36 |
| 12 |
Abrego, Kaylen J (14)
|
GUPACK |
3:22.75 |
3:28.97 |
6.22 |
| 13 |
Clark, Emily K (14)
|
GUSPA |
3:16.18 |
3:43.29 |
27.11 |
| 14 |
To, Logan L (13)
|
GUPACK |
3:36.09 |
3:45.88 |
9.79 |
| 15 |
Gorham, Alexis A (14)
|
GUPFL |
|
3:47.26 |
- |
| 16 |
Granda-Moncayo, Ariana V (13)
|
GUAGS |
|
3:47.40 |
- |
| 17 |
Canny, Addison A (13)
|
GUSPA |
|
3:56.40 |
- |
| 18 |
Flowers, Mia E (14)
|
GUSPA |
|
4:06.30 |
- |
| 19 |
Burns, Gracie I (13)
|
GUPFL |
|
4:08.89 |
- |
| 20 |
Garcia-Lara, Valentina (13)
|
GUPFL |
|
4:23.41 |
- |