| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ceraudo, Savannah G (10)
|
GUSHAC |
30.96 |
29.45 |
-1.51 |
| 2 |
Johnson, Cadence C (9)
|
GUSHAC |
31.27 |
30.30 |
-0.97 |
| 3 |
Ceraudo, Olivia M (10)
|
GUSHAC |
32.87 |
31.29 |
-1.58 |
| 4 |
Turan, Ecem Y (9)
|
GUESCA |
34.31 |
31.77 |
-2.54 |
| 5 |
Elgar, Kate Jasmine E (10)
|
GUSHAC |
34.69 |
32.10 |
-2.59 |
| 6 |
Morgan, Jayde K (9)
|
GUSHAC |
33.79 |
32.54 |
-1.25 |
| 7 |
St John, Laura E (10)
|
GUSHAC |
33.60 |
33.07 |
-0.53 |
| 8 |
Coulon, Myla S (10)
|
GUESCA |
34.00 |
33.72 |
-0.28 |
| 9 |
Coward, Kayin S (10)
|
GUHCAP |
|
34.94 |
- |
| 10 |
Herbst, Grace E (10)
|
GUSHAC |
37.95 |
35.31 |
-2.64 |
| 11 |
Menifee, Mikinzi G (10)
|
GUHCAP |
|
36.59 |
- |
| 12 |
Rodriguez, Bridget A (10)
|
GUHCAP |
39.72 |
38.37 |
-1.35 |
| 13 |
Puente, Allison (10)
|
GUHCAP |
40.66 |
38.58 |
-2.08 |
| 14 |
Lau, Eilene H (9)
|
GUSHAC |
43.47 |
40.33 |
-3.14 |
| 15 |
Krishnan, Mihika H (9)
|
GU300 |
46.00 |
40.68 |
-5.32 |
| 16 |
Lee, Julianne Y (9)
|
GU300 |
|
41.25 |
- |
| 17 |
Sinha, Anisa J (9)
|
GUCOOG |
|
44.31 |
- |
| 18 |
Andreyeva, Aleksandra (10)
|
GUESCA |
|
45.74 |
- |
| 19 |
Herbst, Emily F (9)
|
GUSHAC |
52.67 |
46.30 |
-6.37 |
| 20 |
Marceleno, Valeria (9)
|
GUHCAP |
48.68 |
48.37 |
-0.31 |
| 21 |
Chelius, Naomi T (10)
|
GUCOOG |
1:00.63 |
50.53 |
-10.10 |
| 22 |
Singh, Tvisha (9)
|
GUECS |
|
54.24 |
- |
|
Zhao, Serena (9)
|
GU300 |
35.69 |
NS |
- |
|
Hernandez, Sophia V (9)
|
GUECS |
|
NS |
- |
|
Hinojo, Irais (10)
|
GUESCA |
|
NS |
- |
|
Hinojo, Alani (9)
|
GUESCA |
|
NS |
- |
|
Obua, Cara O (9)
|
GUECS |
48.44 |
NS |
- |