| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vazquez, Michelle (12)
|
GUESA |
2:32.60 |
2:34.49 |
1.89 |
| 2 |
Rodabaugh, Cassandra L (12)
|
GUSWIM |
2:49.14 |
2:45.89 |
-3.25 |
| 3 |
Gutierrez, Riley R (12)
|
GUBLST |
2:52.63 |
2:49.08 |
-3.55 |
| 4 |
Clarke, Callia J (11)
|
GUSPA |
3:01.81 |
2:55.56 |
-6.25 |
| 5 |
Novak, Gabriella M (11)
|
GULSST |
3:05.97 |
2:59.70 |
-6.27 |
| 6 |
Wittmer, Sophie M (12)
|
GUSWIM |
|
3:08.73 |
- |
| 7 |
Reyes, Isabel G (11)
|
GUBLST |
|
3:10.68 |
- |
| 8 |
Montes, Miley (12)
|
GULSST |
|
3:13.33 |
- |
| 9 |
Temiquel, Maddison K (12)
|
GUBLST |
|
3:22.98 |
- |
| 10 |
Fuentes-Pardo, Alejandra N (11)
|
GUESA |
|
3:27.21 |
- |