| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chien, Amber L (12)
|
GUCFSC |
32.18 |
33.12 |
0.94 |
| 2 |
Do, Elizabeth H (11)
|
GUSPA |
40.29 |
40.39 |
0.10 |
| 3 |
Fielding, Isabelle R (12)
|
GUVSST |
44.21 |
40.84 |
-3.37 |
| 4 |
Fawcett, Lillian L (12)
|
GUVSST |
45.92 |
41.97 |
-3.95 |
| 5 |
Curran, Audrey E (12)
|
GUVSST |
41.85 |
42.12 |
0.27 |
| 6 |
Du, Aien (11)
|
GU300 |
42.75 |
42.78 |
0.03 |
| 7 |
Espitia, Sara (12)
|
GUVSST |
44.35 |
42.90 |
-1.45 |
| 8 |
Miller, Brooke C (12)
|
GUUN-V |
46.14 |
43.95 |
-2.19 |
| 9 |
Calayag, Francesca K (11)
|
GUSPA |
44.69 |
43.96 |
-0.73 |
| 10 |
Bhakta, Priyana A (12)
|
GUCFSC |
43.99 |
44.06 |
0.07 |
| 11 |
Nureni-Yusuf, Ajibola F (11)
|
GUCFSC |
47.62 |
46.58 |
-1.04 |
| 12 |
Do, Vivianne V (11)
|
GUSPA |
48.41 |
47.02 |
-1.39 |
| 13 |
Praczko, Victoria (11)
|
GUCFSC |
46.68 |
47.16 |
0.48 |
| 14 |
Herrera, Bianca G (11)
|
GUCFSC |
52.45 |
50.77 |
-1.68 |
| 15 |
Callaci, Ella G (11)
|
GU300 |
|
52.86 |
- |
| 16 |
Mayorca, Concetta V (11)
|
GUCFSC |
|
57.05 |
- |
| 17 |
Schulz, Cori K (12)
|
GUCFSC |
1:14.86 |
1:16.51 |
1.65 |
|
Ellington, Ava C (11)
|
GUCFSC |
50.62 |
NS |
- |