| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Singh, Isabelle H (15)
|
GUPACK |
4:57.39 |
4:56.24 |
-1.15 |
| 2 |
Smith, Megan G (15)
|
GUPACK |
4:46.53 |
5:05.13 |
18.60 |
| 3 |
Gottfried, Charlotte A (16)
|
GUPACK |
4:58.82 |
5:05.54 |
6.72 |
| 4 |
Ryan, Alyssa Q (15)
|
GUPACE |
5:41.23 |
5:06.29 |
-34.94 |
| 5 |
Braden, Paulina N (17)
|
GUPACK |
4:57.15 |
5:10.41 |
13.26 |
| 6 |
Molinaro, Mikaela G (15)
|
GUPACK |
4:59.68 |
5:10.81 |
11.13 |
| 7 |
Criscitiello, Sydney L (17)
|
GUAGS |
5:03.80 |
5:13.00 |
9.20 |
| 8 |
Berkman, Kaylie J (16)
|
GUPFL |
5:26.50 |
5:24.51 |
-1.99 |
| 9 |
Crawford, Cameron R (15)
|
GUPACK |
5:09.30 |
5:25.45 |
16.15 |
| 10 |
Watts, Emma F (15)
|
GUPFL |
|
5:35.46 |
- |
| 11 |
Stewart, Grace I (15)
|
GUTEST |
5:25.17 |
5:43.45 |
18.28 |
| 12 |
Bould, Chelsea E (15)
|
GUPACK |
|
5:46.88 |
- |
| 13 |
Johnson, Averi R (15)
|
GUAGS |
6:12.94 |
6:07.90 |
-5.04 |