| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Garrison, Sophia J (17)
|
STATAC |
30.11 |
27.04 |
-3.07 |
| 2 |
Casten, Genevieve C (15)
|
GUCFSC |
29.06 |
27.85 |
-1.21 |
| 3 |
Eckhart, Keilah E (17)
|
GUCFSC |
31.71 |
27.91 |
-3.80 |
| 4 |
Cates, Tyler K (17)
|
STATAC |
29.60 |
28.77 |
-0.83 |
| 5 |
Zimmerman, Kathryn D (17)
|
GUCFSC |
28.57 |
30.51 |
1.94 |
| 6 |
Hardee, Christin A (18)
|
GUCFSC |
28.85 |
31.25 |
2.40 |
| 7 |
Schlather, Sabrina S (17)
|
GUBATS |
31.21 |
31.85 |
0.64 |
| 8 |
Budsberg, Addison E (16)
|
GUTWST |
36.47 |
33.41 |
-3.06 |
|
Bjelland, Alva (16)
|
GUKATY |
29.32 |
NS |
- |
|
Pulkkinen, Josephine O (15)
|
GUKATY |
31.00 |
NS |
- |
|
Barton, Samantha V (16)
|
GUUN-K |
|
NS |
- |