| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hickok, Jackson J (10)
|
GUUN |
31.90 |
31.45 |
-0.45 |
| 2 |
Vandertie, Crichton J (10)
|
GUUN |
32.33 |
31.59 |
-0.74 |
| 3 |
Cashman, Cullen J (10)
|
NENE |
28.93 |
32.86 |
3.93 |
| 4 |
Nelson, Jaxon (10)
|
GUUN |
33.60 |
33.27 |
-0.33 |
| 5 |
Lopez, Micah (10)
|
ARTEAM |
32.62 |
35.33 |
2.71 |
| 6 |
Mercing, Jagger J (10)
|
ARTEAM |
35.60 |
35.64 |
0.04 |
| 7 |
Zapata, Giovanie (10)
|
FLTV |
36.94 |
35.87 |
-1.07 |
| 8 |
Bianchi, Kai (9)
|
FLFL |
39.30 |
36.39 |
-2.91 |
| 9 |
Rodriguez, Gabrielpaul C (9)
|
GUUN |
36.66 |
36.42 |
-0.24 |
| 10 |
Williams, Emory (10)
|
GUUN |
41.39 |
37.29 |
-4.10 |
| 11 |
Alder, Lincoln D (9)
|
GUUN |
39.00 |
37.77 |
-1.23 |
| 12 |
Carter, Garrett (10)
|
ARTEAM |
38.67 |
39.37 |
0.70 |
| 13 |
Vedder, Thomas B (10)
|
NIUSTA |
41.42 |
40.58 |
-0.84 |
| 13 |
Duncan, Thomas (10)
|
ARTEAM |
1:09.00 |
40.58 |
-28.42 |
| 15 |
Block, Cade (10)
|
SDJRVS |
44.64 |
42.16 |
-2.48 |
| 16 |
Evelsizer, Reese (9)
|
OZSMSC |
46.09 |
42.57 |
-3.52 |
| 17 |
Arnstein, Ethan (10)
|
FLBA |
46.33 |
44.69 |
-1.64 |
|
Spencer, Alistair B (9)
|
GUUN |
39.01 |
NS |
- |