| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Battistic, Mia A (12)
|
GUBLST |
2:39.92 |
2:37.67 |
-2.25 |
| 2 |
Kight, Avery C (12)
|
GUBLST |
2:49.37 |
2:38.92 |
-10.45 |
| 3 |
Damon, Carly E (11)
|
GUSSS |
|
2:43.63 |
- |
| 4 |
Gutierrez, Riley R (11)
|
GUBLST |
|
2:56.34 |
- |
| 5 |
Clepper, Lili G (12)
|
GUSSS |
3:11.34 |
3:03.50 |
-7.84 |
| 6 |
George, Diya H (11)
|
GULIFE |
3:07.49 |
3:04.01 |
-3.48 |
| 7 |
Schuler, Grace E (11)
|
GUSSS |
3:16.59 |
3:08.37 |
-8.22 |
| 8 |
Master, Serenity A (11)
|
GUBLST |
3:35.40 |
3:10.93 |
-24.47 |
| 9 |
Garcia, Ava E (11)
|
GUNOCH |
3:22.21 |
3:16.29 |
-5.92 |
| 10 |
Dominy, Jayla M (11)
|
GUBLST |
|
3:24.20 |
- |
| 11 |
Nwaneri, Jordyn A (11)
|
GUTTST |
|
3:25.07 |
- |
| 12 |
Garcia, Alayda I (11)
|
GUNOCH |
|
3:34.49 |
- |
| 13 |
Zapata, Sophia I (12)
|
GUNOCH |
|
3:47.07 |
- |
| 14 |
Payne, Trinity A (11)
|
GUNOCH |
|
3:58.51 |
- |
|
Hernandez, Arianna I (11)
|
GUNOCH |
|
DQ |
- |