| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Henage, Charles C (10)
|
NCWA |
41.78 |
39.26 |
-2.52 |
| 2 |
Caldwell, Zachery P (10)
|
NCLENR |
42.19 |
41.17 |
-1.02 |
| 3 |
Lindquist, Corbin (10)
|
NCGG |
|
43.37 |
- |
| 4 |
Murphy, Parker O (8)
|
NCNMA |
46.90 |
43.67 |
-3.23 |
| 5 |
Tabor, Beckett C (10)
|
NCGG |
43.14 |
44.28 |
1.14 |
| 6 |
Leckinger, Benjamin T (10)
|
NCNMA |
45.40 |
44.68 |
-0.72 |
| 7 |
Carswell, Nathaniel R (9)
|
NCMSC |
46.15 |
45.35 |
-0.80 |
| 8 |
Gould, Justin P (9)
|
NCNMA |
47.35 |
45.51 |
-1.84 |
| 9 |
Mukkara, Kasyap S (10)
|
NCNMA |
50.39 |
45.61 |
-4.78 |
| 10 |
Hedrick, Noah A (8)
|
NCCVAC |
56.38 |
46.03 |
-10.35 |
| 11 |
Bartlett, Jack W (10)
|
NCCVAC |
50.37 |
47.12 |
-3.25 |
| 12 |
Major, Austin L (9)
|
NCGG |
51.76 |
48.08 |
-3.68 |
| 13 |
Teague, Harrison M (10)
|
NCNMA |
1:05.78 |
48.83 |
-16.95 |
| 14 |
Smith, Gunner L (9)
|
NCCVAC |
|
49.04 |
- |
| 15 |
Ashri, Nikhil (10)
|
NCGG |
51.93 |
49.47 |
-2.46 |
| 16 |
Helf, Evan T (9)
|
NCNMA |
56.27 |
49.81 |
-6.46 |
| 17 |
Arnone, Devon P (10)
|
NCNMA |
48.00 |
50.42 |
2.42 |
| 18 |
Tabor, Noah E (8)
|
NCGG |
51.25 |
51.51 |
0.26 |
| 19 |
Helf, Andrew J (10)
|
NCNMA |
|
54.02 |
- |
| 20 |
Chen, James Z (9)
|
NCNMA |
59.86 |
57.79 |
-2.07 |
| 21 |
Dy, Logan X (9)
|
NCCVAC |
1:09.88 |
59.61 |
-10.27 |
| 22 |
Watson, Ian A (9)
|
NCNMA |
|
1:00.05 |
- |
| 23 |
Kihneman, Max T (7)
|
NCCVAC |
|
1:01.07 |
- |
| 24 |
Perez-Rodriquez, David P (8)
|
NCMSC |
|
1:11.20 |
- |
|
Hashmi, Ahmad H (10)
|
NCNMA |
|
DQ |
- |
|
Olagbemiro, Alexander P (10)
|
NCNMA |
|
DQ |
- |
|
Nagaraj, Chinmay B (9)
|
NCNMA |
|
NS |
- |
|
Prasanth, Adhvay (9)
|
NCNMA |
1:26.16 |
NS |
- |
|
Scott, Landen W (9)
|
NCWST |
52.61 |
NS |
- |