| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rodriquez, Maria V (12)
|
NCMSC |
2:41.29 |
2:38.95 |
-2.34 |
| 2 |
Lawrence, Amelia S (11)
|
NCWA |
2:54.85 |
2:48.05 |
-6.80 |
| 3 |
Murphy, Riley J (12)
|
NCLENR |
2:54.15 |
2:51.05 |
-3.10 |
| 4 |
Collins, Kaci B (11)
|
NCPAC |
3:24.78 |
3:06.49 |
-18.29 |
| 5 |
Soriano, Cierra J (11)
|
NCPAC |
3:39.44 |
3:09.61 |
-29.83 |
| 6 |
Player, Elizabeth J (12)
|
NCSAC |
|
3:16.28 |
- |
| 7 |
Yelton, Lucy E (12)
|
NCMSC |
3:17.66 |
3:17.43 |
-0.23 |
| 8 |
Lopez, Marta B (11)
|
NCMSC |
|
3:20.03 |
- |
| 9 |
White, Skyler R (11)
|
NCCVAC |
|
3:30.54 |
- |
|
Hendricks, Katharyn L (12)
|
NCNMA |
|
NS |
- |
|
Sanchez, Aryanna I (12)
|
NCWA |
|
NS |
- |