| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kunkle, Paige K (14)
|
AMFAST |
2:22.86 |
2:26.44 |
3.58 |
| 2 |
Hunsinger, Delaney L (13)
|
AMFAST |
2:26.22 |
2:26.97 |
0.75 |
| 3 |
Meyers, Mackenzie E (16)
|
AMEFSC |
2:21.95 |
2:29.85 |
7.90 |
| 4 |
Black, Chloe R (15)
|
AMFAST |
2:28.57 |
2:31.23 |
2.66 |
| 5 |
Kurimsky, Kendyl L (13)
|
AMFAST |
2:50.21 |
2:35.15 |
-15.06 |
| 6 |
Geary, Sydney M (16)
|
AMSTEE |
2:23.95 |
2:36.37 |
12.42 |
| 7 |
Catlin, Eliza C (14)
|
AMSTEE |
2:28.67 |
2:38.07 |
9.40 |
| 8 |
Augustynowicz, Macie A (13)
|
AMPTSC |
2:42.06 |
2:40.56 |
-1.50 |
| 9 |
Chen, Ally Y (14)
|
AMPTSC |
2:46.28 |
2:44.15 |
-2.13 |
| 10 |
Economos, Ellora H (13)
|
AMHAAC |
2:56.34 |
2:44.22 |
-12.12 |
| 11 |
Alessio, Madison A (14)
|
AMEFSC |
2:44.77 |
2:44.37 |
-0.40 |
| 12 |
Toth, Sydney L (15)
|
AMEFSC |
2:34.99 |
2:50.06 |
15.07 |
| 13 |
Fine, Elizabeth R (14)
|
AMEFSC |
3:01.24 |
3:04.65 |
3.41 |
| 14 |
Perkins, Ella S (13)
|
AMWAAC |
3:30.36 |
3:08.87 |
-21.49 |
|
Yoder, Ava G (13)
|
AMHAAC |
3:10.66 |
NS |
- |