| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Prenoveau, Kenyon D (17)
|
CTFVYT |
26.38 |
26.67 |
0.29 |
| 2 |
Caldeira, Ava E (13)
|
CTFVYT |
28.57 |
28.29 |
-0.28 |
| 3 |
Corsetti, Brianna R (13)
|
CTIVY |
29.75 |
30.21 |
0.46 |
| 4 |
Herzog, Brooke (13)
|
CTFVYT |
29.79 |
30.27 |
0.48 |
| 5 |
Henry, Taylor L (14)
|
CTFVYT |
29.09 |
30.48 |
1.39 |
| 6 |
Lu, Sandy H (14)
|
CTFVYT |
30.31 |
30.52 |
0.21 |
| 7 |
Harb, Cacia (14)
|
CTFVYT |
30.20 |
30.54 |
0.34 |
| 8 |
Ellsworth, Madison (15)
|
CTFVYT |
30.37 |
30.91 |
0.54 |
| 9 |
Yang, Grace J (13)
|
CTIVY |
31.54 |
31.23 |
-0.31 |
| 10 |
Thompson, Ashley J (17)
|
CTFVYT |
28.97 |
32.83 |
3.86 |
| 11 |
D'Amour, Madelaine C (13)
|
CTIVY |
36.29 |
35.90 |
-0.39 |
| 12 |
Albrecht, Paris C (14)
|
CTFVYT |
36.81 |
37.43 |
0.62 |
| 13 |
Hutchinson, Gabrielle A (14)
|
CTFVYT |
|
40.62 |
- |
|
Manjunath, Rithanya (13)
|
CTIVY |
1:00.83 |
NS |
- |