| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Xu, Ming Na (11)
|
NIFAST |
37.03 |
36.72 |
-0.31 |
| 2 |
Zentner, Sabine E (12)
|
NIHHTA |
47.16 |
42.15 |
-5.01 |
| 3 |
Mount, Reese O (12)
|
NIISWM |
38.11 |
42.46 |
4.35 |
| 4 |
Huyck, Claire (12)
|
NISYCH |
44.02 |
44.13 |
0.11 |
| 5 |
Bebla, Sofia G (12)
|
NISYCH |
38.83 |
45.23 |
6.40 |
| 6 |
Smith, Eva C (11)
|
NIBSSC |
39.20 |
46.04 |
6.84 |
| 7 |
Feng, Sarah (12)
|
NISYCH |
48.96 |
46.05 |
-2.91 |
| 8 |
Stella, Marina C (12)
|
NISYCH |
39.90 |
46.71 |
6.81 |
| 9 |
Romano, Samantha P (12)
|
NISYCH |
43.75 |
47.32 |
3.57 |
| 10 |
Knapp, Riley S (11)
|
NISYCH |
42.33 |
49.32 |
6.99 |
| 11 |
McCormick, Sophie F (12)
|
NIUN-0 |
43.10 |
49.48 |
6.38 |
| 12 |
Borkson, Sasha M (12)
|
NIISWM |
42.86 |
50.54 |
7.68 |
| 13 |
Partlow, Mckenzie S (11)
|
NIOLSC |
43.03 |
51.32 |
8.29 |
| 14 |
Commandeur, Sophia H (12)
|
NISYCH |
48.99 |
52.94 |
3.95 |
| 15 |
Conzone, Emily E (11)
|
NIOLSC |
45.26 |
55.44 |
10.18 |
| 16 |
Lopez, Sofia H (12)
|
NIGRSC |
51.18 |
56.19 |
5.01 |
| 17 |
Stokes-Rees, Margaret B (11)
|
NISYCH |
|
1:05.07 |
- |
|
O'Brien, Allison C (11)
|
NIHHTA |
41.93 |
NS |
- |