| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ramsay, Auston J (16)
|
NESSYS |
48.33 |
47.87 |
-0.46 |
| 2 |
Romanowski, Hunter (17)
|
NJMB |
49.23 |
48.68 |
-0.55 |
| 3 |
Watler, Jaleel C (17)
|
MRNYCC |
49.44 |
49.55 |
0.11 |
| 4 |
Malone, Christian J (16)
|
CTFVYM |
49.77 |
49.71 |
-0.06 |
| 5 |
Clough, Michael J (15)
|
NIBAAC |
49.48 |
49.76 |
0.28 |
| 6 |
Horbacz, Justin M (17)
|
NJMB |
49.56 |
49.81 |
0.25 |
| 7 |
Karangekis, Mitchell S (16)
|
CTOAK |
51.36 |
50.08 |
-1.28 |
| 8 |
Souza, Zachary H (17)
|
NESSYS |
50.12 |
50.11 |
-0.01 |
| 9 |
Burr, Trenton H (13)
|
MRHAA |
50.65 |
50.73 |
0.08 |
| 10 |
Riozzi-Bodine, Ethan J (15)
|
NJMB |
49.61 |
50.81 |
1.20 |
| 11 |
Maida, Troy A (16)
|
MRHAA |
49.50 |
51.01 |
1.51 |
| 12 |
Howard, Ben R (17)
|
MRHAA |
51.00 |
51.35 |
0.35 |
| 13 |
Cross, Maxwell R (16)
|
NEMST |
51.50 |
51.53 |
0.03 |
| 14 |
McKelvey, Dylan L (17)
|
NJMB |
51.33 |
52.65 |
1.32 |
| 15 |
Chang, Oscar (15)
|
MRHAA |
53.00 |
53.09 |
0.09 |