| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cheney, Brielle B (12)
|
MYST |
34.37 |
34.72 |
0.35 |
| 2 |
Goodwill, Alli (12)
|
COR |
34.76 |
36.03 |
1.27 |
| 3 |
Moats, Taylor (12)
|
COR |
36.52 |
37.42 |
0.90 |
| 4 |
Canfield, Alyson (12)
|
YJET |
39.27 |
39.48 |
0.21 |
| 5 |
Bland, Skyler L (12)
|
MYST |
39.88 |
39.87 |
-0.01 |
| 6 |
Lucks, Summer (12)
|
WRN |
40.52 |
40.08 |
-0.44 |
| 7 |
Soler-Harand, Pauline (12)
|
NIGRAY |
42.03 |
40.63 |
-1.40 |
| 8 |
Newton, Lilly (11)
|
WRN |
42.00 |
41.16 |
-0.84 |
| 9 |
Crosscut, Madison N (11)
|
COUN |
40.83 |
41.64 |
0.81 |
| 10 |
Dvorak, Evabelle (12)
|
COUN |
43.60 |
42.04 |
-1.56 |
| 11 |
Crocker, Jenna (11)
|
TYST |
41.85 |
42.09 |
0.24 |
| 12 |
Lamont, Lydia (11)
|
LOCK |
42.30 |
42.92 |
0.62 |
| 13 |
Lumia, Elsa (12)
|
YJET |
44.70 |
43.31 |
-1.39 |
| 14 |
Ongley, Lauren (11)
|
TYST |
39.77 |
43.83 |
4.06 |
| 15 |
Colwell, Sara (12)
|
COR |
41.48 |
44.00 |
2.52 |
| 15 |
Gullace, Charlotte (12)
|
NIGRAY |
42.41 |
44.00 |
1.59 |
| 17 |
Bulger, Olivia (11)
|
ZZESYS |
45.73 |
44.12 |
-1.61 |
| 18 |
Rospierski, Rachel (11)
|
ZZESYS |
|
44.38 |
- |
| 19 |
Enderton, Lauren (11)
|
LOCK |
43.34 |
45.02 |
1.68 |
| 20 |
Hess, Ali (12)
|
COUN |
45.56 |
45.18 |
-0.38 |
| 21 |
Faulk, Morgan (12)
|
YJET |
43.58 |
45.53 |
1.95 |
| 22 |
Williams, Sabrina (12)
|
NIBRAD |
44.44 |
46.01 |
1.57 |
| 23 |
Bish, Avery A (11)
|
MYST |
45.88 |
46.20 |
0.32 |
| 24 |
Brook, Eliza (12)
|
WRN |
48.44 |
46.21 |
-2.23 |
| 25 |
Dixon, Emilee (12)
|
OCY |
47.51 |
47.43 |
-0.08 |
| 26 |
Waite, Leah (11)
|
YJET |
48.06 |
48.61 |
0.55 |
| 27 |
Conroe, Annie (12)
|
YJET |
50.25 |
50.27 |
0.02 |
| 28 |
Johnson, Madeline (11)
|
TYST |
50.74 |
50.85 |
0.11 |
| 29 |
Lindstrom, Kelly (12)
|
YJET |
59.72 |
1:02.47 |
2.75 |
|
Clark, Alijanayah (11)
|
YJET |
52.16 |
DQ |
- |
|
Hogan, Lily (11)
|
COR |
|
DQ |
- |