| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mohr, Grace K (13)
|
NCTYDE |
34.91 |
38.00 |
3.09 |
| 2 |
Ross, Christina N (14)
|
NCLOY |
40.68 |
41.08 |
0.40 |
| 3 |
Chance, Kristin T (14)
|
NCRSA |
38.63 |
42.41 |
3.78 |
| 4 |
McChesney, Shelby K (13)
|
NCTYDE |
47.17 |
42.75 |
-4.42 |
| 5 |
Kightlinger, Emma F (14)
|
NCRSA |
45.33 |
46.60 |
1.27 |
| 6 |
Love, Samantha D (14)
|
NCHPSC |
50.84 |
49.84 |
-1.00 |
| 7 |
Blackwell, Waverly C (13)
|
NCHPSC |
50.25 |
50.66 |
0.41 |
| 8 |
Shaver, Madison B (14)
|
NCRACY |
|
52.61 |
- |
| 9 |
Darby, Taylor L (13)
|
NCRACY |
56.71 |
54.42 |
-2.29 |
| 10 |
Ontiveros, Valeria (13)
|
NCHPSC |
45.99 |
56.14 |
10.15 |
| 11 |
Wainright, Maris O (13)
|
NCRACY |
53.89 |
58.87 |
4.98 |
|
Thompson, Mary K (14)
|
NCHPSC |
46.72 |
NS |
- |
|
Klosterman, Carstyn J (14)
|
NCTYDE |
45.52 |
NS |
- |